Ovocleidin (OC 116) Is Present in Avian Skeletal Tissues
نویسندگان
چکیده
منابع مشابه
Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116.
The role of avian eggshell matrix proteins in shell formation is poorly understood. This calcitic biomaterial forms in a uterine fluid where the protein composition varies during the initial, calcification, and terminal phases of eggshell deposition. A specific antibody was raised to a 116-kDa protein, which is most abundant in uterine fluid during active eggshell calcification. This antiserum ...
متن کاملAn Efficient Method for Co-purification of Eggshell Matrix Proteins OC-17, OC-116, and OCX-36
In this study, we improved the eggshell-membrane separation process by separating the shell and membrane with EDTA solution, evaluating effects of three different extraction solutions (acetic acid, EDTA, and phosphate solution), and co-purifying multiple eggshell proteins with two successive ion-exchange chromatography procedures (CM Sepharose Fast Flow and DEAE Sepharose Fast Flow). The recove...
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Background: The present study aimed to investigate the presence of leptin mRNA transcript in epididymal spermatozoa of Holstein cattle by RT-PCR analysis. Materials and Methods: To collect sperm from epididymis, the whole testes of three adult Holstein bulls were quickly removed after slaughtering. Samples were transferred to the laboratory on ice and subsequently the epididymal contents were c...
متن کاملImmunostaining of skeletal tissues.
Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralised tissues is more complicated than with soft tissues. This can in most cases be overcome by demineralisin...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 2008
ISSN: 0032-5791
DOI: 10.3382/ps.2008-00031